For more detailed explanations and recommendations about the preparation and submission of samples, please refer to the following link: (301) 251-1007 customerservice@psomagen.com: New York 760 Parkside Ave. Suite 120 Brooklyn, NY 11226 Please contact us for any inquiry. Macrogen's NGS technology is able to sequence and quantify all the small RNA families in a sample and profile the miRNA, siRNA, piRNA and other non-coding RNAs. It includes Metagenome Sequencing. Sequencing Platforms • Illumina HiSeq 4000 / HiSeq 2500 Macrogen Sequencing Troubleshooting guide: Most of sequencing failure is caused by sample condition or the specificity of composition, in this case there is no possibility of improvement through any other approach use, and therefore it is not the case of reanalysis Macrogen sequencing policy. All of the process from sample receipt to data dispatch are done under the tight control of our own LIMS which is developed by us. We will continue working towards sequencing and will communicate during the progress, until the delivery of the result. Macorgen shall not be liable for any loss caused by commodity transaction or leading and borrowing money through service between users and a third party and expected profit from service. For sequencing of tubes or plates, click on Standard-seq. For plate orders, here is a helpful guide for using the Macrogen online template. Maryland 1330 Piccard Drive Suite 205 Rockville, MD 20850 Tel. Macrogen have no obligation to confirm and represent any opinion and information provided by Macrogen’ sequencing service, users and third party. In other words, it is used to determine which microbial communities are present in a sample collected in a certain environment, and to identify their interactions and roles. It is used primarily to analyze the distribution and types of bacteria and fungi. Sequencing Sample/Primer Requirements If the TE buffer with EDTA (concentration of 0.1-1Mm) is used for dissolving DNA, EDTA take up free magnesium ions, which reduces DNA polymerase activity resulting in sequencing reaction failure. Nuclease-Free super low EDTA TE buffer (10mM Tris-HCI, pH8.0, 0.01mM EDTA) can be used but with caution. Metagenome sequencing is a method of identifying microbial communities that exist in different environments. Sample Macrogen Type of Analysis De novo assembly,Annotation Type of Sequencer PacBio RSII ... what sequencing enzyme and chemistry were used, sample name and ... performs high quality de novo assembly using a single PacBio library preparation. Step 2: When your samples are ready for sequencing, login to your customer account on the Macrogen website and place your order. In most circumstance, library preparation/QC will take place unless sample QC has failed. The high sensitivity of scRNA seq requires careful processing of the sample to ensure optimal results. Macrogen sequencing service is provided by 3730XL DNA analyzer(AB,USA) and BigDye v3.1(AB,USA). See here for the requirements of sample preparation, also shown briefly below. Macrogen Europe B.V provides a high quality DNA Sequencing service for academics, government, research institutions and private companies at Very Competitive Price. Macrogen Europe B.V offers DNA sequencing using state-of-the-art robotics and instrumentation to generate high quality sequence data at affordable prices. Working towards sequencing and will communicate during the progress, until the delivery of result. Of sample preparation, also shown briefly below When your samples are ready sequencing., 0.01mM EDTA ) can be used but with caution service, users and third party click... Qc has failed see here for the requirements of sample preparation, also briefly! 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